Setdb1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Setdb1^{tm1c(EUCOMM)Wtsi}
• Name: SET domain, bifurcated 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6188271
• Synonyms: ESET^Flox
• Gene: Setdb1 Location: Chr3:95230836-95264513 bp, - strand Genetic Position: Chr3, 40.74 cM, cytoband F2
• Alliance: Setdb1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Setdb1 gene page

Mutation origin

• Mutant Cell Line: EPD0028_1_B07
• Germline Transmission: Earliest citation of germline transmission: J:257670
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 95256909 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 95257725. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:257670)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Setdb1 Mutation: 58 strains or lines available

References

• Original: J:257670 Yang L, et al., Generation and characterization of mice with mesenchyme-specific deletion of the entire ESET histone methyltransferase protein. Genesis. 2018 Feb;56(2)
• All: 6 reference(s)