Kdm4b^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kdm4b^{tm1d(EUCOMM)Wtsi}
• Name: lysine (K)-specific demethylase 4B; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6188079
• Gene: Kdm4b Location: Chr17:56633062-56709870 bp, + strand Genetic Position: Chr17, 29.27 cM
• Alliance: Kdm4b^{tm1d(EUCOMM)Wtsi} page
• IMPC: Kdm4b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262875
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt0 cassette was inserted at position 56662423 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 56663316. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:262875)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm4b Mutation: 54 strains or lines available

References

• Original: J:262875 Cheng Y, et al., KDM4B protects against obesity and metabolic dysfunction. Proc Natl Acad Sci U S A. 2018 Jun 12;115(24):E5566-E5575
• All: 1 reference(s)