Tg(Slc6a3-aop3*,-EGFP)11Gfng
Transgene Detail

Summary

• Symbol: Tg(Slc6a3-aop3*,-EGFP)11Gfng
• Name: transgene insertion 11, Guoping Feng
• MGI ID: MGI:6187695
• Synonyms: DAT-eArchT3.0-EGFP, Slc6a3-eArchT3.0-EGFP
• Transgene: Tg(Slc6a3-aop3*,-EGFP)11Gfng Location: unknown
• Alliance: Tg(Slc6a3-aop3*,-EGFP)11Gfng page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: BAC RP24-269I17 was used to obtain entire Slc6a3 locus; the following was inserted in-frame immediately after the Slc6a3 start codon: an eArchT3.0 cDNA sequence (described further below), a viral 2A oligopeptide sequence, an enhanced green fluorescent protein (EGFP), a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE; to enhance mRNA stability) and a bovine growth hormone polyadenylation signal (BGHpA). The eArchT3.0 sequence is a mammalian codon-optimized cDNA encoding ArchT (the Archaerhodopsin-3 protein [aop3] derived from the halophilic bacterium Halorubrum strain TP009) that was further modified to have a neurite/membrane-trafficking signal (TS) sequence (amino acids GEYIPLDQIDINV) and an endoplasmic reticulum (ER) exporting sequence (amino acids FCYENEV). (J:341572)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:341572 Krol A, et al., Two eARCHT3.0 Lines for Optogenetic Silencing of Dopaminergic and Serotonergic Neurons. Front Neural Circuits. 2019;13:4
• All: 1 reference(s)