Gt(ROSA)26Sor^{tm2(CAG-Mef2a*)Thbr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-Mef2a*)Thbr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Thomas Braun
• MGI ID: MGI:6164311
• Synonyms: Mef2A-tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-Mef2a*)Thbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262205
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(CAG-Mef2a*)Thbr} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mef2a (MGI:99532)

• Mutation details: A CAGGS promoter, floxed neo resistance and polyA cassettes, and mutant Mef2a cDNA lacking coding exon 2 were inserted via homologous recombination. Deletion of exon 2 removes the Mir133a and Mir1a target sites. (J:262205)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:262205 Wust S, et al., Metabolic Maturation during Muscle Stem Cell Differentiation Is Achieved by miR-1/133a-Mediated Inhibition of the Dlk1-Dio3 Mega Gene Cluster. Cell Metab. 2018 May 1;27(5):1026-1039.e6
• All: 1 reference(s)