Dars2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dars2^{tm1c(KOMP)Wtsi}
• Name: aspartyl-tRNA synthetase 2 (mitochondrial); targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6163613
• Synonyms: Dars2^loxP
• Gene: Dars2 Location: Chr1:160868171-160898228 bp, - strand Genetic Position: Chr1, 69.75 cM
• Alliance: Dars2^{tm1c(KOMP)Wtsi} page
• IMPC: Dars2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210635
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 160892554 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 160893282. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:210635)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dars2 Mutation: 36 strains or lines available

References

• Original: J:210635 Dogan SA, et al., Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart. Cell Metab. 2014 Mar 4;19(3):458-69
• All: 7 reference(s)