Mybpc3^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Mybpc3^{tm1c(EUCOMM)Hmgu}
• Name: myosin binding protein C, cardiac; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6160321
• Synonyms: Mybpc3^flox
• Gene: Mybpc3 Location: Chr2:90948489-90966861 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E1
• Alliance: Mybpc3^{tm1c(EUCOMM)Hmgu} page
• IMPC: Mybpc3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262067
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 91119563 of Chromosome 2 into intron 2 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted into intron 5 at position 91120795. Exons 3-5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:262067)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mybpc3 Mutation: 41 strains or lines available

References

• Original: J:262067 Matsuyama S, et al., Interaction between cardiac myosin-binding protein C and formin Fhod3. Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4386-E4395
• All: 3 reference(s)