Zfp36^tm3.1Pjb
Targeted Allele Detail

Summary

• Symbol: Zfp36^tm3.1Pjb
• Name: zinc finger protein 36; targeted mutation 3.1, Paul J Blackshear
• MGI ID: MGI:6159268
• Synonyms: C116R
• Gene: Zfp36 Location: Chr7:28076208-28078680 bp, - strand Genetic Position: Chr7, 16.72 cM
• Alliance: Zfp36^tm3.1Pjb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:261625
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site was inserted upstream of exon 2 and an FRT flanked neo cassette, loxP site and mutant exon 2 with a T377C mutation resulting in a C116R substitution were inserted downstream of exon 2 via homologous recombination. Cre mediated recombination removed exon 2 and the neo cassette. The mutation in exon 2 is in the first zinc finger and abrogates binding to RNA in in vitro assays. Expression levels of the mutant protein are similar to wild-type expression levels. (J:261625)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp36 Mutation: 17 strains or lines available

References

• Original: J:261625 Lai WS, et al., A Knock-In Tristetraprolin (TTP) Zinc Finger Point Mutation in Mice: Comparison with Complete TTP Deficiency. Mol Cell Biol. 2018 Feb 15;38(4)
• All: 1 reference(s)