Ube3a^em1Yelg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ube3a^em1Yelg
• Name: ubiquitin protein ligase E3A; endonuclease-mediated mutation 1, Ype Elgersma
• MGI ID: MGI:6158669
• Synonyms: Ube3a^mIso3
• Gene: Ube3a Location: Chr7:58878498-58961284 bp, + strand Genetic Position: Chr7, 33.95 cM
• Alliance: Ube3a^em1Yelg page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: An ATG codon (Chromosome 7: 59,228,802-59,311,536; Ube3a exon 4/5) encoding the initiating methionine of UBE3A isoform 3 was mutated into an alanine (GCG) using CRISPR/Cas9, such that only isoform 2 is generated in these mice. A diagnostic silent DraIII restriction site was also introduced in exon 4. (J:261868)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube3a Mutation: 72 strains or lines available

References

• Original: J:261868 Elgersma Y, Alleles of Ube3a. MGI Direct Data Submission. 2018
• All: 3 reference(s)