Col1a1^{tm13(CAG-Nfib_v3,-luc2)Nki}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm13(CAG-Nfib_v3,-luc2)Nki}
• Name: collagen, type I, alpha 1; targeted mutation 13, The Netherlands Cancer Institute
• MGI ID: MGI:6158302
• Synonyms: invCAG-Nfib_v3-Luc
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm13(CAG-Nfib_v3,-luc2)Nki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238585
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: RMCE retargeted Col1a1^tm2Nki to replace the frt-flanked PGK-Neo cassette in the 3' UTR of the Col1a1 by a "Flp-in" plasmid encoding a pFrt-invCAG-Nfib_v3-Luc inversion transgene, called inv-Cag-Luc2, by co-transfected with a Flpe-expressing plasmid to facilitate recombination into the 3' UTR of the Col1a1 locus. This transgene contains the constitutive CAGGs promoter that drives the expression of the Hygromycin-resistance gene, present in the targeted Col1a1 locus. Upon expression of the Cre recombinase, the promoter is inverted due to flanking mutant loxP sites, i.e. lox66 and lox71, placing the promoter in front of the firefly Luciferase2 gene and the cDNA for the short variant lacking exon 9-11 (variant 3) of Nfib. (J:238585)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

Parental ES cells were derived from FVB.129P2-Trp53^tm1Brn Rb1^tm2Brn mice and containing in addition to the targeted Col1a1^tm2Nki allele.

References

• Original: J:238585 Semenova EA, et al., Transcription Factor NFIB Is a Driver of Small Cell Lung Cancer Progression in Mice and Marks Metastatic Disease in Patients. Cell Rep. 2016 Jul 19;16(3):631-43
• All: 1 reference(s)