Tg(Olfr73,-EGFP)AKtou
Transgene Detail

Summary

• Symbol: Tg(Olfr73,-EGFP)AKtou
• Name: transgene insertion A, Kazushige Touhara
• MGI ID: MGI:6157860
• Synonyms: mOR-EG-IRES-gapEGFP, TgA
• Transgene: Tg(Olfr73,-EGFP)AKtou Location: unknown
• Alliance: Tg(Olfr73,-EGFP)AKtou page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2J)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The transgene is comprised of an 8.0 kb genomic fragment comprising the sequences between 5.6 kb upstream of the Olfr73 start codon, and the PvuII site in the 3 untranslated region. A SacII-NotI site was engineered downstream of the Olfr73 stop codon, into which a IRES-gapEGFP was inserted, which encodes an internal ribosome entry site (IRES) and a fusion protein consisting of the N-terminal 20 amino acids of Gap43 and EGFP. Three lines of mice expressing the transgene in different patterns were generated, TgA, TgB and TgC. (J:117260)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:117260 Oka Y, et al., Odorant receptor map in the mouse olfactory bulb: in vivo sensitivity and specificity of receptor-defined glomeruli. Neuron. 2006 Dec 7;52(5):857-69
• All: 8 reference(s)