Gramd2a^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gramd2a^em1(IMPC)Tcp
• Name: GRAM domain containing 2A; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:6156594
• Gene: Gramd2a Location: Chr9:59587427-59626157 bp, + strand Genetic Position: Chr9, 32.05 cM
• Alliance: Gramd2a^em1(IMPC)Tcp page
• IMPC: Gramd2a gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project TCPR0844 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of ACAAGGCCCCCTTCTCAAGC and GATACTTGGGCAGGACTCAA targeting the 5' side and GGTACCCGCGTAGGTAAGCT and CAATCTAGATCCTCTTCTCG targeting the 3' side leading to a 821-bp deletion from Chr9:59709401 to 59710221 (GRCm38). (J:237616)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gramd2a Mutation: 10 strains or lines available

References

• Original: J:237616 MGI and IMPC, MGI Curation of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). MGI Direct Data Submission. 2017-8
• All: 2 reference(s)