Iars1^{em1(IMPC)Wtsi}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Iars1^{em1(IMPC)Wtsi}
• Name: isoleucyl-tRNA synthetase 1; endonuclease-mediated mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:6153367
• Gene: Iars1 Location: Chr13:49835606-49887743 bp, + strand Genetic Position: Chr13, 25.38 cM
• Alliance: Iars1^{em1(IMPC)Wtsi} page
• IMPC: Iars1 gene page

Mutation origin

• Strain of Origin: C57BL/6N
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at Wellcome Sanger Institute using Cas9 and guides with spacer sequences ACCAACAAGCTCCCAAATGG, CGTAGAGGTCTGCATCTGAA, GTATCCAGTTGTTCCCAACA, and TGGTACAACCACTCGCTGCA that targeted exon(s) ENSMUSE00000274328.4. This resulted in deletion(s) of 5 bp (location: Chr13:49844844-49844848; GRCm39), 375 bp (location: Chr13:49844853-49845227; GRCm39). This description was generated automatically. Additional molecular information including FASTA sequence support for this allele can be found here: (IMPC Gene page) and viewed in a genome context here: (IMPC Genome Browser). (J:384794)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Iars1 Mutation: 81 strains or lines available

References

• Original: J:237616 MGI and IMPC, MGI Curation of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). MGI Direct Data Submission. 2017-8
• All: 2 reference(s)