Dagla^tm1.2Zim
Targeted Allele Detail

Summary

• Symbol: Dagla^tm1.2Zim
• Name: diacylglycerol lipase, alpha; targeted mutation 1.2, Andreas Zimmer
• MGI ID: MGI:6153198
• Gene: Dagla Location: Chr19:10222629-10282241 bp, - strand Genetic Position: Chr19, 6.55 cM
• Alliance: Dagla^tm1.2Zim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:250058
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 1 and a FRT-flanked neomycin resistance gene and a second loxP site were inserted downstream of exon 1. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination excised exon 1. Real-time PCR confirmed complete loss of mRNA transcript and immunohistochemistry confirmed the absence of protein in brain. (J:250058)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dagla Mutation: 60 strains or lines available

References

• Original: J:250058 Jenniches I, et al., Anxiety, Stress, and Fear Response in Mice With Reduced Endocannabinoid Levels. Biol Psychiatry. 2016 May 15;79(10):858-868
• All: 1 reference(s)