Pou2f1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pou2f1^{tm1c(EUCOMM)Wtsi}
• Name: POU domain, class 2, transcription factor 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6151070
• Gene: Pou2f1 Location: Chr1:165692723-165830247 bp, - strand Genetic Position: Chr1, 73.21 cM
• Alliance: Pou2f1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Pou2f1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228515
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 165736170 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 165737062. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:228515)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pou2f1 Mutation: 98 strains or lines available

References

• Original: J:228515 Shakya A, et al., Oct1 and OCA-B are selectively required for CD4 memory T cell function. J Exp Med. 2015 Nov 16;212(12):2115-31
• All: 5 reference(s)