Usp22^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Usp22^{tm1c(KOMP)Wtsi}
• Name: ubiquitin specific peptidase 22; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6150921
• Synonyms: usp22^fl
• Gene: Usp22 Location: Chr11:61042611-61065881 bp, - strand Genetic Position: Chr11, 37.96 cM
• Alliance: Usp22^{tm1c(KOMP)Wtsi} page
• IMPC: Usp22 gene page

Mutation origin

• Mutant Cell Line: EPD0119_2_D11
• Germline Transmission: Earliest citation of germline transmission: J:260101
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 61058865 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 61059653. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:260101)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Usp22 Mutation: 31 strains or lines available

References

• Original: J:260101 Li C, et al., The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining. Nat Commun. 2018 Mar 8;9(1):1006
• All: 9 reference(s)