Spp1^tm1.1Mlsh
Targeted Allele Detail

Summary

• Symbol: Spp1^tm1.1Mlsh
• Name: secreted phosphoprotein 1; targeted mutation 1.1, Mari L Shinohara
• MGI ID: MGI:6150284
• Synonyms: LSL^fl-delta45Spp1
• Gene: Spp1 Location: Chr5:104582984-104588916 bp, + strand Genetic Position: Chr5, 50.68 cM
• Alliance: Spp1^tm1.1Mlsh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:259332
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Modified isoform(s), Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced with an FRT-flanked STOP cassette and a modified exon 2 lacking the 45 nucleotides correspondihng to the signal sequence. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of exon 3. Cre-mediated recombination is required to achieve expression of the intracellular isoform of the protein. This allele cannot produce the soluble isoform. (J:259332)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spp1 Mutation: 45 strains or lines available

References

• Original: J:259332 Kanayama M, et al., Skewing of the population balance of lymphoid and myeloid cells by secreted and intracellular osteopontin. Nat Immunol. 2017 Sep;18(9):973-984
• All: 2 reference(s)