Ncor2^tm1.1Laz
Targeted Allele Detail

Summary

• Symbol: Ncor2^tm1.1Laz
• Name: nuclear receptor co-repressor 2; targeted mutation 1.1, Mitchell A Lazar
• MGI ID: MGI:6149745
• Synonyms: S-DADm, SMRT^Y470A
• Gene: Ncor2 Location: Chr5:125094217-125256283 bp, - strand Genetic Position: Chr5, 63.96 cM, cytoband F
• Alliance: Ncor2^tm1.1Laz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252085
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Single point mutation
• Mutation details: A Y470A mutation was introduced into exon 13 of the locus, which is within the deacetylase activation domain of the protein product. A floxed neomycin selection cassette was also placed about downstream from the targeted exon. Founder mice derived from correctly targeted ES cells were crossed with EIIa-cre transgenic mice to remove the neomycin selection cassette. The introduced mutation was predicted to disrupt interactions between the Ncor2 protein product and the histone deacetylase Hdac3. Expression of the mutant allele was similar to endogenous expression as determined by immunoblot analysis of homozygous liver extracts. The mutant Ncor2 protein failed to interact with Hdac3 protein as determined by immunoprecipitation assays. (J:252085)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncor2 Mutation: 170 strains or lines available

References

• Original: J:252085 You SH, et al., Nuclear receptor co-repressors are required for the histone-deacetylase activity of HDAC3 in vivo. Nat Struct Mol Biol. 2013 Feb;20(2):182-7
• All: 6 reference(s)