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Krastm1.1Khai
Targeted Allele Detail
Nomenclature
Symbol: Krastm1.1Khai
Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 1.1, Kevin Haigis
MGI ID: MGI:6149500
Synonyms: KrasLSL-A146T
Gene: Kras  Location: Chr6:145216699-145250239 bp, - strand  Genetic Position: Chr6, 77.37 cM, cytoband G2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:276349
Parent Cell Line:  TL1/TL-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Conditional ready, Humanized sequence)
Mutation:    Nucleotide substitutions
 
Mutation detailsThe targeting vector is designed to insert a loxP-flanked STOP cassette downstream of exon 1 of the Kras gene. Exon 4 was modified to harbor a missense mutation at codon 146, resulting in an alanine to threonine mutation (A146T) commonly found in patients with cancers of the gastrointestinal tract and blood. An FRT-flanked neomycin resistance (neo) cassette is placed directly downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:276349)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 6 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Kras Mutation:  41 strains or lines available
References
Original:  J:276349 Poulin EJ, et al., Tissue-Specific Oncogenic Activity of KRAS(A146T). Cancer Discov. 2019 Jun;9(6):738-755
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/30/2021
MGI 6.16
The Jackson Laboratory