Slc32a1^tm2.1Yyan
Targeted Allele Detail

Summary

• Symbol: Slc32a1^tm2.1Yyan
• Name: solute carrier family 32 (GABA vesicular transporter), member 1; targeted mutation 2.1, Yuchio Yanagawa
• MGI ID: MGI:6148233
• Synonyms: VGAT^flox
• Gene: Slc32a1 Location: Chr2:158452687-158457668 bp, + strand Genetic Position: Chr2, 78.72 cM, cytoband H1-3
• Alliance: Slc32a1^tm2.1Yyan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241981
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 3. The neomycin selection cassette was removed via FLP-mediated recombination. This creates a conditional-ready allele with exons 2 and 3 floxed and without the neomycin selection cassette. (J:241981)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc32a1 Mutation: 55 strains or lines available

References

• Original: J:241981 Kayakabe M, et al., Motor dysfunction in cerebellar Purkinje cell-specific vesicular GABA transporter knockout mice. Front Cell Neurosci. 2013;7:286
• All: 3 reference(s)