Sqstm1^tm2Keta
Targeted Allele Detail

Summary

• Symbol: Sqstm1^tm2Keta
• Name: sequestosome 1; targeted mutation 1, Keiji Tanaka
• MGI ID: MGI:6147658
• Synonyms: Sqstm1-GFP^KI, Sqstm1^tm1Kmts, Sqstm1^tm2(GFP)Keta
• Gene: Sqstm1 Location: Chr11:50090193-50101654 bp, - strand Genetic Position: Chr11, 30.36 cM, cytoband B1.2
• Alliance: Sqstm1^tm2Keta page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233914
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector inserted a cDNA fragment encoded by exons 3-8 (302-1326) of Sqstm1 fused to GFP cDNA, a poly(A) signal sequence, and a neo resistance gene cassette after exon 2 of the endogenous gene. (J:233914)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sqstm1 Mutation: 32 strains or lines available

References

• Original: J:233914 Eino A, et al., Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. J Cell Sci. 2015 Dec 1;128(23):4453-61
• All: 2 reference(s)