Ndrg4^tm1.2Scba
Targeted Allele Detail

Summary

• Symbol: Ndrg4^tm1.2Scba
• Name: N-myc downstream regulated gene 4; targeted mutation 1.2, Scott Baldwin
• MGI ID: MGI:6147566
• Gene: Ndrg4 Location: Chr8:96403602-96441584 bp, + strand Genetic Position: Chr8, 47.12 cM
• Alliance: Ndrg4^tm1.2Scba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233338
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was introduced into intron 5 and a loxP site followed by a FRT-flanked PGK-neomycin resistance cassette were inserted into intron 7. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination deleted exons 5-7 in the germline. Western blot analysis confirmed absence of protein expression in the brain and heart. (J:233338)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ndrg4 Mutation: 19 strains or lines available

References

• Original: J:233338 Qu X, et al., Postnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice. Biochem Biophys Res Commun. 2016 Feb 12;470(3):613-9
• All: 2 reference(s)