Cnnm4^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cnnm4^{tm1b(EUCOMM)Wtsi}
• Name: cyclin M4; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:6144058
• Synonyms: Cnnm4^delta
• Gene: Cnnm4 Location: Chr1:36510701-36547845 bp, + strand Genetic Position: Chr1, 15.28 cM, cytoband B
• Alliance: Cnnm4^{tm1b(EUCOMM)Wtsi} page
• IMPC: Cnnm4 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246429
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 36497561 of Chromosome 1 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 36500237. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:246429)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnnm4 Mutation: 20 strains or lines available

References

• Original: J:246429 Yamazaki D, et al., The Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction. J Cell Sci. 2016 May 01;129(9):1940-9
• All: 3 reference(s)