Astl^em1Dean
Endonuclease-mediated Allele Detail

Summary

• Symbol: Astl^em1Dean
• Name: astacin like metalloendopeptidase; endonuclease-mediated mutation 1, Jurrien Dean
• MGI ID: MGI:6143846
• Synonyms: Astl^delta
• Gene: Astl Location: Chr2:127180559-127199571 bp, + strand Genetic Position: Chr2, 61.93 cM, cytoband F
• Alliance: Astl^em1Dean page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The 13 amino acid cortical granule localizing motif was disrupted by the deletion of the sequences encoding the first 7 aa (52-DKDIPAI-58) encoded entirely by exon 2 with a protospacer adjacent motif (PAM) sequence. The resulting expressed protein lacked the amino acids 52-58. (J:239512)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Astl Mutation: 23 strains or lines available

References

• Original: J:239512 Xiong B, et al., A Unique Egg Cortical Granule Localization Motif Is Required for Ovastacin Sequestration to Prevent Premature ZP2 Cleavage and Ensure Female Fertility in Mice. PLoS Genet. 2017 Jan;13(1):e1006580
• All: 1 reference(s)