Slc2a2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc2a2^{tm1c(KOMP)Wtsi}
• Name: solute carrier family 2 (facilitated glucose transporter), member 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6121049
• Synonyms: Slc2a2^FLOX
• Gene: Slc2a2 Location: Chr3:28752052-28782510 bp, + strand Genetic Position: Chr3, 12.19 cM
• Alliance: Slc2a2^{tm1c(KOMP)Wtsi} page
• IMPC: Slc2a2 gene page

Mutation origin

• Mutant Cell Line: EPD0207_2_B08
• Germline Transmission: Earliest citation of germline transmission: J:240599
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 28767360 of Chromosome 3 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 28768253. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele, removing the lacZ and neomycin cassettes and leaving exon 4 floxed. (J:240599)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc2a2 Mutation: 31 strains or lines available

References

• Original: J:240599 Schmitt CC, et al., Intestinal invalidation of the glucose transporter GLUT2 delays tissue distribution of glucose and reveals an unexpected role in gut homeostasis. Mol Metab. 2017 Jan;6(1):61-72
• All: 3 reference(s)