Pbrm1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pbrm1^{tm1c(EUCOMM)Wtsi}
• Name: polybromo 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6120907
• Gene: Pbrm1 Location: Chr14:30741095-30843549 bp, + strand Genetic Position: Chr14, 19.09 cM, cytoband B
• Alliance: Pbrm1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Pbrm1 gene page

Mutation origin

• Mutant Cell Line: EPD0089_3_C01
• Germline Transmission: Earliest citation of germline transmission: J:254910
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 30748980 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 30749852. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:254910)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pbrm1 Mutation: 91 strains or lines available

References

• Original: J:254910 Espana-Agusti J, et al., Loss of PBRM1 rescues VHL dependent replication stress to promote renal carcinogenesis. Nat Commun. 2017 Dec 11;8(1):2026
• All: 1 reference(s)