Mfsd2a^em1Chgu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mfsd2a^em1Chgu
• Name: MFSD2 lysolipid transporter A, lysophospholipid; endonuclease-mediated mutation 1, Chenghua Gu
• MGI ID: MGI:6120542
• Synonyms: Mfsd2a^D96A
• Gene: Mfsd2a Location: Chr4:122840643-122854981 bp, - strand Genetic Position: Chr4, 57.34 cM, cytoband D1
• Alliance: Mfsd2a^em1Chgu page

Mutation origin

• Strain of Origin: (C57BL/6J x SJL/J)F1/J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: An A to C mutation resulting in a D96A substitution was inserted in exon 3 by CRISPR/Cas9 mediated recombination. Immunohistochemical and western blot analysis indicated no change in protein expression in the brains of homozygous mice compared to wild-type controls. Altered lipid profiles in the brains of homozygotes indicate this mutation abolishes the lipid transport function of the gene. (J:256030)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mfsd2a Mutation: 40 strains or lines available

References

• Original: J:256030 Andreone BJ, et al., Blood-Brain Barrier Permeability Is Regulated by Lipid Transport-Dependent Suppression of Caveolae-Mediated Transcytosis. Neuron. 2017 May 3;94(3):581-594.e5
• All: 1 reference(s)