Gt(ROSA)26Sor^{tm2(tTA,tetO-APOE_i4,-EGFP)Gbu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(tTA,tetO-APOE_i4,-EGFP)Gbu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Guojun Bu
• MGI ID: MGI:6119699
• Synonyms: iE4
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(tTA,tetO-APOE_i4,-EGFP)Gbu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256115
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(tTA,tetO-APOE_i4,-EGFP)Gbu} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

  The APOE 4 isoform is only expressed after Cre-mediated recombination

• Mutation details: A construct consisting of a floxed neo cassette, tTA site, a CMV promoter with a tetracycline responsive element, a human APOE_i4 cDNA, an IRES site and an EGFP gene, was inserted via homologous recombination. In the absence of Cre-mediated recombination the human protein is not expressed. Following Cre-mediated recombination the human protein is expressed and this expression may be suppressed with the addition of doxycycline. (J:256115)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:256115 Liu CC, et al., ApoE4 Accelerates Early Seeding of Amyloid Pathology. Neuron. 2017 Dec 6;96(5):1024-1032.e3
• All: 4 reference(s)