Gt(ROSA)26Sortm1(tTA,tetO-APOE_i3,-EGFP)Gbu
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm1(tTA,tetO-APOE_i3,-EGFP)Gbu |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Guojun Bu |
MGI ID: |
MGI:6119698 |
Synonyms: |
iE3 |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm1(tTA,tetO-APOE_i3,-EGFP)Gbu page
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Germline Transmission: |
Earliest citation of germline transmission:
J:256115
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted (Conditional ready, Inducible, Inserted expressed sequence, Reporter) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Gt(ROSA)26Sortm1(tTA,tetO-APOE_i3,-EGFP)Gbu expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
APOE (348) |
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The APOE 3 isoform is only expressed after Cre-mediated recombination |
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Mutation details: A construct consisting of a floxed neo cassette, tTA site, a CMV promoter with a tetracycline responsive element, a human APOE_i3 cDNA, an IRES site and an EGFP gene, was inserted via homologous recombination. In the absence of Cre-mediated recombination the human protein is not expressed. Following Cre-mediated recombination the human protein is expressed and this expression may be suppressed with the addition of doxycycline.
(J:256115)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gt(ROSA)26Sor Mutation: |
942 strains or lines available
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Original: |
J:256115 Liu CC, et al., ApoE4 Accelerates Early Seeding of Amyloid Pathology. Neuron. 2017 Dec 6;96(5):1024-1032.e3 |
All: |
3 reference(s) |
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