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Dbhem1.1(flpo)Rray
Endonuclease-mediated Allele Detail
Nomenclature
Symbol: Dbhem1.1(flpo)Rray
Name: dopamine beta hydroxylase; endonuclease-mediated mutation 1.1, Russell Ray
MGI ID: MGI:6118955
Synonyms: DBH-FLPo, Dbhtm1(DBH-FLPo)Rray
Gene: Dbh  Location: Chr2:27165507-27183204 bp, + strand  Genetic Position: Chr2, 19.29 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:251840
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprtb-m2
Mutation
description
Allele Type:    Endonuclease-mediated (Null/knockout, Recombinase)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe mouse line was generated by homologous recombination using the CRISPR/Cas9 technology. The coding sequences for optimized FLP recombinase was inserted at the transcriptional start site of the endogenous Dbh gene replacing 106 bps of the first exon; a single lox2722 (modified LoxP) site marks the former position of a floxed neomycin resistance cassette that was deleted by Cre-mediated recombination. The resulting allele is a knock-out of Dbh protein product but express Flpo recombinase under control of the Dbh gene. (J:251840)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Dbh     Summary of all recombinase alleles driven by Dbh. Comparative matrix view of recombinase activities
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dbh Mutation:  9 strains or lines available
References
Original:  J:251840 Sun JJ, et al., Generation of Two Noradrenergic-Specific Dopamine-Beta-Hydroxylase-FLPo Knock-In Mice Using CRISPR/Cas9-Mediated Targeting in Embryonic Stem Cells. PLoS One. 2016;11(7):e0159474
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
07/29/2020
MGI 6.15
The Jackson Laboratory