Trp73^tm1.2Piet
Targeted Allele Detail

Summary

• Symbol: Trp73^tm1.2Piet
• Name: transformation related protein 73; targeted mutation 1.2, Jennifer A Pietenpol
• MGI ID: MGI:6117398
• Synonyms: p73^-
• Gene: Trp73 Location: Chr4:154140706-154224332 bp, - strand Genetic Position: Chr4, 83.79 cM
• Alliance: Trp73^tm1.2Piet page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234561
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 7 and an FRT flanked neomycin resistance gene cassette and loxP site were inserted downstream of exon 9. The neomycin cassette was removed through subsequent Flp-mediated recombination. The floxed exons 7-9 were deleted via Cre-mediated recombination. Western blot analysis confirmed the absence of full-length protein and the presence of a truncated protein lacking exons 7-9. This truncated version which lacks part of the DNA-binding domain, nuclear localization signal, and oligomerization domain, is functionally inactive as a transcription factor. (J:234561)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp73 Mutation: 47 strains or lines available

References

• Original: J:234561 Marshall CB, et al., p73 Is Required for Multiciliogenesis and Regulates the Foxj1-Associated Gene Network. Cell Rep. 2016 Mar 15;14(10):2289-300
• All: 2 reference(s)