Ano2^tm1Lyj
Targeted Allele Detail

Summary

• Symbol: Ano2^tm1Lyj
• Name: anoctamin 2; targeted mutation 1, Lily Jan
• MGI ID: MGI:6116817
• Synonyms: TMEM16B KO
• Gene: Ano2 Location: Chr6:125667382-126017089 bp, + strand Genetic Position: Chr6, 59.58 cM
• Alliance: Ano2^tm1Lyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256160
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129 x C57BL/6)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding sequence for farnesylated mCherry (mCherry-F, membrane bound form) was inserted in-frame at the alternative start site in exon 3 of the gene. Reduced expression of the full length wild type mRNA in the IO nucleus, as well as the retina, olfactory bulb, and hippocampus was observed. Homozygous mutant mice displayed no detectable wild type protein expression in the IO nucleus while membrane-associated mCherry-F expression was seen in cells that normally express the endogenous protein. (J:256160)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ano2 Mutation: 57 strains or lines available

References

• Original: J:256160 Zhang Y, et al., Inferior Olivary TMEM16B Mediates Cerebellar Motor Learning. Neuron. 2017 Aug 30;95(5):1103-1111.e4
• All: 5 reference(s)