Pkn1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pkn1^{tm1c(KOMP)Wtsi}
• Name: protein kinase N1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6116286
• Synonyms: Pkn1^f
• Gene: Pkn1 Location: Chr8:84393165-84425808 bp, - strand Genetic Position: Chr8, 40.22 cM
• Alliance: Pkn1^{tm1c(KOMP)Wtsi} page
• IMPC: Pkn1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256597
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 84417387 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 84418271. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:256597)

Tumor Data

List all tumor models in MMHCdb carrying Pkn1^{tm1c(KOMP)Wtsi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pkn1 Mutation: 45 strains or lines available

References

• Original: J:256597 Yang CS, et al., The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression. Prostate. 2017 Nov;77(15):1452-1467
• All: 2 reference(s)