Gt(ROSA)26Sor^{tm12(Tcf/Lef-GFP*)Nat}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm12(Tcf/Lef-GFP*)Nat}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 12, Jeremy Nathans
• MGI ID: MGI:6116237
• Synonyms: Rosa26 Tcf./Lef-LSL-H2B-GFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm12(Tcf/Lef-GFP*)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256044
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced into the locus an FRT-flanked neomycin resistance cassette, eight tandem repeats of the Lef/Tcf binding site (CCTTTGATC), a minumal heat shock 68 promoter, floxed stop cassette, a histone H2B-GFP-6xMyc fusion protein and bovine growth hormonr 3'UTR. (J:256044)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:256044 Cho C, et al., Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation. Neuron. 2017 Aug 30;95(5):1056-1073.e5
• All: 7 reference(s)