Slc6a14^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc6a14^{tm1d(KOMP)Wtsi}
• Name: solute carrier family 6 (neurotransmitter transporter), member 14; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6115852
• Gene: Slc6a14 Location: ChrX:21581139-21608597 bp, + strand Genetic Position: ChrX, 16.78 cM, cytoband A2
• Alliance: Slc6a14^{tm1d(KOMP)Wtsi} page
• IMPC: Slc6a14 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256446
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 21590422 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 21591293. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:256446)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc6a14 Mutation: 14 strains or lines available

References

• Original: J:256446 Di Paola M, et al., SLC6A14 Is a Genetic Modifier of Cystic Fibrosis That Regulates Pseudomonas aeruginosa Attachment to Human Bronchial Epithelial Cells. mBio. 2017 Dec 19;8(6)
• All: 1 reference(s)