Gpc4tm2c(EUCOMM)Wtsi
Targeted Allele Detail
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Symbol: |
Gpc4tm2c(EUCOMM)Wtsi |
Name: |
glypican 4; targeted mutation 2c, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:6115608 |
Gene: |
Gpc4 Location: ChrX:51141898-51254129 bp, - strand Genetic Position: ChrX, 28.33 cM
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Alliance: |
Gpc4tm2c(EUCOMM)Wtsi page
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IMPC: |
Gpc4 gene page |
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Germline Transmission: |
Earliest citation of germline transmission:
J:256085
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready) |
Mutation: |
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Insertion
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Mutation details: The L1L2_Bact_P cassette was inserted at position 51162887 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 51164045. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s).
(J:256085)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gpc4 Mutation: |
18 strains or lines available
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Original: |
J:256085 Farhy-Tselnicker I, et al., Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation. Neuron. 2017 Oct 11;96(2):428-445.e13 |
All: |
1 reference(s) |
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