Irak3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Irak3^{tm1c(EUCOMM)Wtsi}
• Name: interleukin-1 receptor-associated kinase 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6115588
• Synonyms: IRAK-M^fl
• Gene: Irak3 Location: Chr10:119977553-120038035 bp, - strand Genetic Position: Chr10, 67.94 cM, cytoband D2
• Alliance: Irak3^{tm1c(EUCOMM)Wtsi} page
• IMPC: Irak3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256180
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 120014378 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 120015162. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:256180)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Irak3 Mutation: 104 strains or lines available

References

• Original: J:256180 Jiang D, et al., A novel mouse model of conditional IRAK-M deficiency in myeloid cells: application in lung Pseudomonas aeruginosa infection. Innate Immun. 2017 Feb;23(2):206-215
• All: 1 reference(s)