Th^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Th^{tm1c(KOMP)Wtsi}
• Name: tyrosine hydroxylase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6115504
• Synonyms: TH^fl
• Gene: Th Location: Chr7:142446516-142453732 bp, - strand Genetic Position: Chr7, 88.06 cM
• Alliance: Th^{tm1c(KOMP)Wtsi} page
• IMPC: Th gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255933
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 142448725 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 142449655. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:255933)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Th Mutation: 58 strains or lines available

References

• Original: J:255933 Stephens SBZ, et al., Effects of Selective Deletion of Tyrosine Hydroxylase from Kisspeptin Cells on Puberty and Reproduction in Male and Female Mice. eNeuro. 2017 May-Jun;4(3):ENEURO.0150-17.2017
• All: 1 reference(s)