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Targeted Allele Detail
Symbol: Ngly1tm2d(KOMP)Wtsi
Name: N-glycanase 1; targeted mutation 2d, Wellcome Trust Sanger Institute
MGI ID: MGI:6114996
Gene: Ngly1  Location: Chr14:6157837-6220449 bp, - strand  Genetic Position: Chr14, 7.08 cM, cytoband A2
Mutant Cell Line:  EPD0044_5_F07
Germline Transmission:  Earliest citation of germline transmission: J:101977
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion     Vector: L1L2_gt2
Mutation detailsThe L1L2_gt2 cassette was placed upstream of the critical exon (exon 2) of the gene; inserting at bp position Chr14:16,254,154 [Build GRCm38]. The cassette is composed of composed of an FRT site, a mouse En2 intron/splice-acceptor/exon (En2 SA), a self-cleaving 2A peptide, the beta-galactosidase gene (lacZ), a second self-cleaving 2A peptide, the neomycin-resistance gene (neo), an SV40 polyadenylation signal (SV40pA), a second FRT site and a loxP site. A second loxP site was inserted downstream of exon 2 at bp position Chr14:16,255,007 bp [Build GRCm38]; thereby flanking exon 2 with loxP sites. Cre-mediated recombination removed exon 2. Flp-mediated recombination removed the FRT-flanked neo cassette (J:101977, J:148605)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ngly1 Mutation:  30 strains or lines available
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory