Gt(ROSA)26Sor^{tm1(CAG-cas9*,-BFP)Khk}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-cas9*,-BFP)Khk}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Klaus H Kaestner
• MGI ID: MGI:6114620
• Synonyms: Gt(ROSA)26Sor[tm1(CAG-dCas9-SunTag)Khk]
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-cas9*,-BFP)Khk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255667
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Endonuclease, Epitope tag)
• Mutation: Insertion
• Mutation details: The pAi9-dCas9 targeting construct introduced into the locus a CAG promoter, floxed stop cassette, neomycin selection cassette, a nuclease dead cas9 sequence tagged with 10 copies of the GCN4 peptide v4 (SUperNova; SunTag), 2A sequence and BFP reporter gene. (J:255667)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:255667 Wangensteen KJ, et al., Combinatorial genetics in liver repopulation and carcinogenesis with a novel in vivo CRISPR activation platform. Hepatology. 2017 Nov 1
• All: 2 reference(s)