Tcrb^Ln4Sdou
Allele Detail

Summary

• Symbol: Tcrb^Ln4Sdou
• Name: T cell receptor beta chain; recombination line 4, Stephanie Dougan
• MGI ID: MGI:6113684
• Synonyms: 615 TCRbeta
• Gene: Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C

Mutation origin

• Strain of Origin: (C57BL/6 x 129)F1

Mutation description

• Allele Type: Other (Not Specified)
• Mutation: Other
• Mutation details: This allele is generated using a somatic cell nuclear transfer (SCNT). Male agouti (C57BL/6 x 129)F1 mice were immunized with palmitoylated TRYP1 (tyrosinase-related protein or TRP1) peptide, anti-CD40 antibody and poly-IC to create a population of TRP1 specific lymphocytes. This peptide-based vaccine elicits a robust population of tetramer+ CD8+ T cells that can reject established B16 tumors. Six to eight days post-immunization, TRP1 tetramer+ populations were observed in the spleen. Using flow cytometry, CD8+ TRP1 tetramer+ populations were isolated and individual donor nuclei selected and placed into an enucleated oocyte. Cells were developed to the blastocyst stage and used to generate embryonic stem (ES) cells. ES cells were injected into recipient blastocysts, and blastocysts implanted into pseudopregnant females. Tcrb was sequenced and identified as V12-1*01 J1-2*01 D1*01 and propagated. (J:254784)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tcrb Mutation: 95 strains or lines available

References

• Original: J:254784 Dougan SK, et al., Transnuclear TRP1-specific CD8 T cells with high or low affinity TCRs show equivalent antitumor activity. Cancer Immunol Res. 2013 Aug;1(2):99-111
• All: 2 reference(s)