Penk^{tm1.1(cre)Gsche}
Targeted Allele Detail

Summary

• Symbol: Penk^{tm1.1(cre)Gsche}
• Name: preproenkephalin; targeted mutation 1.1, Gregory Scherrer
• MGI ID: MGI:6113637
• Synonyms: Penk^cre
• Gene: Penk Location: Chr4:4133536-4138815 bp, - strand Genetic Position: Chr4, 2.31 cM
• Alliance: Penk^{tm1.1(cre)Gsche} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:253245
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac or C57BL/6J

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Penk^{tm1.1(cre)Gsche} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Penk (MGI:104629)

• Mutation details: The targeting vector was constructed using recombineering techniques. An 8,528 bp genomic DNA fragment containing the last exon of the mouse Penk gene was retrieved from BAC clone RP23-RP23- 365K8 to a vector containing the DT gene. A cassette of IRES-Cre frt-PGKNeo-frt was inserted between stop codon TGA and 3' UTR. The neo cassette was removed by breeding germline chimeras with FLP1 expressing homozygous females. (J:253245)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Penk (mouse) Summary of all recombinase alleles driven by Penk. Comparative matrix view of recombinase activities Alliance mouse Penk gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Penk Mutation: 21 strains or lines available

Notes

"The targeting vector was electroporated into F1 hybrid of 129S6 x C57BL/6J ES cells derived by Janelia Transgenic Facility." (J:253245)

References

• Original: J:253245 Francois A, et al., A Brainstem-Spinal Cord Inhibitory Circuit for Mechanical Pain Modulation by GABA and Enkephalins. Neuron. 2017 Feb 22;93(4):822-839.e6
• All: 2 reference(s)