Dnmt3a^tm1Rlvn
Targeted Allele Detail

Summary

• Symbol: Dnmt3a^tm1Rlvn
• Name: DNA methyltransferase 3A; targeted mutation 1, Ross Levine
• MGI ID: MGI:6113600
• Synonyms: Dnmt3a^LSL-R878H, Dnmt3a^R878H
• Gene: Dnmt3a Location: Chr12:3856007-3964443 bp, + strand Genetic Position: Chr12, 1.99 cM, cytoband A2-A3
• Alliance: Dnmt3a^tm1Rlvn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241153
• Parent Cell Line: iTL IC1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, No functional change)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector is designed to insert a minigene combining exons 23 and 24 in place of the endogenous exon 23. This minigene was modified to harbor a guanine to adenine substitution at amino acid 878, resulting in an arginine to histidine mutation (R878H), which corresponds to a R882H mutation commonly found in patients with acute myeloid leukemia (AML). A floxed STOP/neomycin cassette, including a splice acceptor and 3x SV40 polyA signals (saPGK-Neo-pA), was placed directly upstream of the modified minigene. (J:241153)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dnmt3a Mutation: 140 strains or lines available

References

• Original: J:241153 Guryanova OA, et al., DNMT3A mutations promote anthracycline resistance in acute myeloid leukemia via impaired nucleosome remodeling. Nat Med. 2016 Dec;22(12):1488-1495
• All: 8 reference(s)