Mfsd2b^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mfsd2b^{tm1c(KOMP)Wtsi}
• Name: MFSD2 lysolipid transporter B, sphingolipid; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6111979
• Synonyms: Mfsd2b^f
• Gene: Mfsd2b Location: Chr12:4912440-4924359 bp, - strand Genetic Position: Chr12, 2.11 cM
• Alliance: Mfsd2b^{tm1c(KOMP)Wtsi} page
• IMPC: Mfsd2b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:250123
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 4918712 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 4919873. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:250123)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mfsd2b Mutation: 39 strains or lines available

References

• Original: J:250123 Vu TM, et al., Mfsd2b is essential for the sphingosine-1-phosphate export in erythrocytes and platelets. Nature. 2017 Oct 26;550(7677):524-528
• All: 4 reference(s)