Kras^{tm5(Hras*G12V)Bbd}
Targeted Allele Detail

Summary

• Symbol: Kras^{tm5(Hras*G12V)Bbd}
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 5, Mariano Barbacid
• MGI ID: MGI:6111952
• Synonyms: K^HRasV12, K-Ras^LSLH-RasG12V
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^{tm5(Hras*G12V)Bbd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239406
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Kras^{tm5(Hras*G12V)Bbd} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hras (MGI:96224)	Codon 12 contains a Gly to Val mutation.

• Mutation details: Murine Hras cDNA with glycine codon 12 (GGA) changed to valine (GTA) (p.G12V) was knocked in replacing the coding region of exon 2. The Hras cDNA is preceded by an Lox-STOP-Lox cassette. (J:239406)

Tumor Data

List all tumor models in MMHCdb carrying Kras^{tm5(Hras*G12V)Bbd}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 76 strains or lines available

References

• Original: J:239406 Drosten M, et al., H-Ras and K-Ras Oncoproteins Induce Different Tumor Spectra When Driven by the Same Regulatory Sequences. Cancer Res. 2017 Feb 01;77(3):707-718
• All: 1 reference(s)