Tbc1d4^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Tbc1d4^{tm1c(EUCOMM)Hmgu}
• Name: TBC1 domain family, member 4; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6111786
• Synonyms: AS160^f
• Gene: Tbc1d4 Location: Chr14:101679796-101846627 bp, - strand Genetic Position: Chr14, 50.9 cM
• Alliance: Tbc1d4^{tm1c(EUCOMM)Hmgu} page
• IMPC: Tbc1d4 gene page

Mutation origin

• Mutant Cell Line: HEPD0601_5_F08
• Germline Transmission: Earliest citation of germline transmission: J:246944
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 101726727 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 101727865. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:246944)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbc1d4 Mutation: 50 strains or lines available

References

• Original: J:246944 Xie B, et al., The Inactivation of RabGAP Function of AS160 Promotes Lysosomal Degradation of GLUT4 and Causes Postprandial Hyperglycemia and Hyperinsulinemia. Diabetes. 2016 Nov;65(11):3327-3340
• All: 1 reference(s)