D130043K22Rik^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: D130043K22Rik^{tm1c(KOMP)Wtsi}
• Name: RIKEN cDNA D130043K22 gene; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6111776
• Synonyms: Kiaa0319-Flx
• Gene: D130043K22Rik Location: Chr13:25029118-25085253 bp, + strand Genetic Position: Chr13, 10.72 cM
• Alliance: D130043K22Rik^{tm1c(KOMP)Wtsi} page
• IMPC: D130043K22Rik gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241324
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 25047100 of Chromosome 13 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 25047964. Exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 6. (J:241324)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any D130043K22Rik Mutation: 59 strains or lines available

References

• Original: J:241324 Martinez-Garay I, et al., Normal radial migration and lamination are maintained in dyslexia-susceptibility candidate gene homolog Kiaa0319 knockout mice. Brain Struct Funct. 2017 Apr;222(3):1367-1384
• All: 3 reference(s)