Cdkal1^{tm2c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cdkal1^{tm2c(EUCOMM)Wtsi}
• Name: CDK5 regulatory subunit associated protein 1-like 1; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6111542
• Synonyms: Cdkal1^flox
• Gene: Cdkal1 Location: Chr13:29375729-30039657 bp, - strand Genetic Position: Chr13, 12.93 cM, cytoband A3.3
• Alliance: Cdkal1^{tm2c(EUCOMM)Wtsi} page
• IMPC: Cdkal1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:253165
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30029923 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 30030809. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:253165)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdkal1 Mutation: 56 strains or lines available

References

• Original: J:253165 Palmer CJ, et al., Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue. Mol Metab. 2017 Oct;6(10):1212-1225
• All: 1 reference(s)