Card14^tm1.1Abow
Targeted Allele Detail

Summary

• Symbol: Card14^tm1.1Abow
• Name: caspase recruitment domain family, member 14; targeted mutation 1.1, Anne M Bowcock
• MGI ID: MGI:6111507
• Synonyms: Card14^LSL-E138A
• Gene: Card14 Location: Chr11:119198594-119236201 bp, + strand Genetic Position: Chr11, 83.36 cM
• Alliance: Card14^tm1.1Abow page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:291840
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, No functional change)
• Mutations: Insertion, Single point mutation
• Mutation details: The allele with floxed minigene (exons 4-22) encoding the wild type C-terminus of Card14 has been inserted between exons 3 and 4 of the Card14 locus and an E138A mutation introduced into endogenous exon 5, was generated by Taconic. In the absence of Cre-mediated recombination, wild type Card14 is expressed from the early endogenous exons (starting from exon 3), and exons within the minigene. A 3xFLAG tag has been inserted at the end of the minigene to produce C-terminally 3x FLAG-tagged wild type CARD14. A STOP and human growth hormone poly (A) (hGHpA) cassette prevent aberrant read-through to the remaining endogenous locus. After Cre-mediated recombination, the minigene is excised, allowing transcription of Card14 from the endogenous exons and expression of CARD14[E138A]. The neo was removed via crossing with FLP deleter mice. (J:291840)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Card14 Mutation: 38 strains or lines available

References

• Original: J:291840 Manils J, et al., CARD14(E138A) signalling in keratinocytes induces TNF-dependent skin and systemic inflammation. Elife. 2020 Jun 29;9:e56720
• All: 1 reference(s)