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Endonuclease-mediated Allele Detail
Symbol: Gt(ROSA)26Sorem1(CAG-cas9*,-EGFP)Dvs
Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, David V Serreze
MGI ID: MGI:6111351
Synonyms: Rosa26CAG-LSL-Cas9-P2A-EGFP
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Strain of Origin:  NOD/ShiLtJ
Allele Type:    Endonuclease-mediated (Conditional ready, Endonuclease, Reporter)
Mutation:    Insertion
Mutation detailsSequences include CMV enhancer/chicken beta-actin promoter (CAG), a loxP-STOP(3xSV40 polyA)-loxP cassette (LSL), a 3xFLAG sequence, a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]), a P2A ribosomal skip cleavage peptide sequence, an enhanced green fluorescent protein gene (EGFP), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) and a bovine growth hormone polyA sequence (BGHpA). To facilitate insertion into the Gt(ROSA)26Sor locus on chromosome 6 via homology directed repair (HDR), the LSL-Cas9-P2A-EGFP sequences were co-injected with a large piece of Gt(ROSA)26Sor locus homologous DNA. (J:101977)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  741 strains or lines available
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
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