Tcte1^em1Mngx
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tcte1^em1Mngx
• Name: t-complex-associated testis expressed 1; endonuclease-mediated mutation 1, Mingxi Liu
• MGI ID: MGI:6111214
• Synonyms: Tcte1^FLAG2, Tcte1^tm3(Mngx)
• Gene: Tcte1 Location: Chr17:45834360-45853605 bp, + strand Genetic Position: Chr17, 22.54 cM, cytoband C
• Alliance: Tcte1^em1Mngx page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Using an sgRNA and CRISPR/Cas9 technology, a FLAG sequence with a 5' GGATCC (Gly-Ser) spacer was inserted at the 3' end of the coding sequence immediately upstream of the stop codon. Donor ssDNA, sgRNA and Cas9 mRNA were injected into zygotes. (J:243766)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcte1 Mutation: 29 strains or lines available

References

• Original: J:243766 Castaneda JM, et al., TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa. Proc Natl Acad Sci U S A. 2017 Jul 03;114(27):E5370-E5378
• All: 1 reference(s)